SiRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells.

ABSTRACT: The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct l

SUBMITTER: Andersen MO 

PROVIDER: S-EPMC2990512 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature