Molecular characterization of the skate peripherin/rds gene: relationship to its orthologues and paralogues.

ABSTRACT:

Purpose

A great deal of information about functionally significant domains of a protein may be obtained by comparison of primary sequences of gene homologues over a broad phylogenetic base. This study was designed to identify evolutionarily conserved domains of the photoreceptor disc membrane protein peripherin/rds by analysis of the homologue in a primitive vertebrate, the skate.

Methods

A skate retinal cDNA library was screened using a mouse peripherin/rds clone. The 5' and 3' untranslated regions of the skate peripherin/rds (srds) cDNA were isolated by the rapid amplification of cDNA ends (RACE) approach. The gene structure was characterized by PCR amplification and sequencing of genomic fragments. Northern and Western blot analyses were used to identify srds transcript a

SUBMITTER: Li C 

PROVIDER: S-EPMC2991160 | biostudies-literature | 2003 Jun

REPOSITORIES: biostudies-literature