Designing and engineering evolutionary robust genetic circuits.

ABSTRACT:

Background

One problem with engineered genetic circuits in synthetic microbes is their stability over evolutionary time in the absence of selective pressure. Since design of a selective environment for maintaining function of a circuit will be unique to every circuit, general design principles are needed for engineering evolutionary robust circuits that permit the long-term study or applied use of synthetic circuits.

Results

We first measured the stability of two BioBrick-assembled genetic circuits propagated in Escherichia coli over multiple generations and the mutations that caused their loss-of-function. The first circuit, T9002, loses function in less than 20 generations and the mutation that repeatedly causes its loss-of-function is a deletion between two homologous tra

SUBMITTER: Sleight SC 

PROVIDER: S-EPMC2991278 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature