The insulator protein SU(HW) fine-tunes nuclear lamina interactions of the Drosophila genome.

ABSTRACT: Specific interactions of the genome with the nuclear lamina (NL) are thought to assist chromosome folding inside the nucleus and to contribute to the regulation of gene expression. High-resolution mapping has recently identified hundreds of large, sharply defined lamina-associated domains (LADs) in the human genome, and suggested that the insulator protein CTCF may help to demarcate these domains. Here, we report the detailed structure of LADs in Drosophila cells, and investigate the putative roles of five insulator proteins in LAD organization. We found that the Drosophila genome is also organized in discrete LADs, which are about five times smaller than human LADs but contain on average a similar number of genes. Systematic comparison to new and published insulator binding maps shows tha

SUBMITTER: van Bemmel JG 

PROVIDER: S-EPMC2991331 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature