A sandwiched microarray platform for benchtop cell-based high throughput screening.

ABSTRACT: The emergence of combinatorial chemistries and the increased discovery of natural compounds have led to the production of expansive libraries of drug candidates and vast numbers of compounds with potentially interesting biological activities. Despite broad interest in high throughput screening (HTS) across varied fields of biological research, there has not been an increase in accessible HTS technologies. Here, we present a simple microarray sandwich system suitable for screening chemical libraries in cell-based assays at the benchtop. The microarray platform delivers chemical compounds to isolated cell cultures by 'sandwiching' chemical-laden arrayed posts with cell-seeded microwells. In this way, an array of sealed cell-based assays was generated without cross-contamination between neigh

SUBMITTER: Wu J 

PROVIDER: S-EPMC2991467 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature