MTOR-mediated activation of p70 S6K induces differentiation of pluripotent human embryonic stem cells.

ABSTRACT: Deciding to exit pluripotency and undergo differentiation is of singular importance for pluripotent cells, including embryonic stem cells (ESCs). The molecular mechanisms for these decisions to differentiate, as well as reversing those decisions during induced pluripotency (iPS), have focused largely on transcriptomic controls. Here, we explore the role of translational control for the maintenance of pluripotency and the decisions to differentiate. Global protein translation is significantly reduced in hESCs compared to their differentiated progeny. Furthermore, p70 S6K activation is restricted in hESCs compared to differentiated fibroblast-like cells. Disruption of p70 S6K-mediated translation by rapamycin or siRNA knockdown in undifferentiated hESCs does not alter cell viability or expre

SUBMITTER: Easley CA 

PROVIDER: S-EPMC2993047 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature