Matrix metalloproteinase-9 and cell division in neuroblastoma cells and bone marrow macrophages.

ABSTRACT: Matrix metalloproteinases (MMPs) degrade the extracellular matrix and carry out key functions in cell development, cancer, injury, and regeneration. In addition to its well recognized extracellular action, functional intracellular MMP activity under certain conditions is supported by increasing evidence. In this study, we observed higher gelatinase activity by in situ zymography and increased MMP-9 immunoreactivity in human neuroblastoma cells and in bone marrow macrophages undergoing mitosis compared with resting cells. We studied the pattern of immunoreactivity at the different stages of cell division by confocal microscopy. Immunostaining with different monoclonal antibodies against MMP-9 revealed a precise, dynamic, and well orchestrated localization of MMP-9 at the different stages of

SUBMITTER: Sans-Fons MG 

PROVIDER: S-EPMC2993298 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature