MicroRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell disease.

ABSTRACT: Although individuals with homozygous sickle cell disease (HbSS) share the same genetic mutation, the severity and manifestations of this disease are extremely heterogeneous. We have previously shown that the microRNA expression in normal and HbSS erythrocytes exhibit dramatic differences. In this study, we identify a subset of HbSS patients with higher erythrocytic miR-144 expression and more severe anemia. HbSS erythrocytes are known to have reduced tolerance for oxidative stress, yet the basis for this phenotype remains unknown. This study reveals that miR-144 directly regulates nuclear factor-erythroid 2-related factor 2, a central regulator of cellular response to oxidative stress, and modulates the oxidative stress response in K562 cell line and primary erythroid progenitor cells. We

SUBMITTER: Sangokoya C 

PROVIDER: S-EPMC2993631 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature