Rational design of modular circuits for gene transcription: A test of the bottom-up approach.

ABSTRACT:

Background

Most of synthetic circuits developed so far have been designed by an ad hoc approach, using a small number of components (i.e. LacI, TetR) and a trial and error strategy. We are at the point where an increasing number of modular, inter-changeable and well-characterized components is needed to expand the construction of synthetic devices and to allow a rational approach to the design.

Results

We used interchangeable modular biological parts to create a set of novel synthetic devices for controlling gene transcription, and we developed a mathematical model of the modular circuits. Model parameters were identified by experimental measurements from a subset of modular combinations. The model revealed an unexpected feature of the lactose repressor system, i.e. a residu

SUBMITTER: Ceroni F 

PROVIDER: S-EPMC2993646 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature