A rapid and simple method for constructing stable mutants of Acinetobacter baumannii.

ABSTRACT:

Background

Acinetobacter baumannii is a multidrug-resistant bacterium responsible for nosocomial infections in hospitals worldwide. Study of mutant phenotypes is fundamental for understanding gene function. The methodologies developed to inactivate A. baumannii genes are complicated and time-consuming; sometimes result in unstable mutants, and do not enable construction of double (or more) gene knockout mutant strains of A. baumannii.

Results

We describe here a rapid and simple method of obtaining A. baumannii mutants by gene replacement via double crossover recombination, by use of a PCR product that carries an antibiotic resistance cassette flanked by regions homologous to the target locus. To demonstrate the reproducibility of the approach, we produced mutants of three di

SUBMITTER: Aranda J 

PROVIDER: S-EPMC2993698 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature