Disabled-2 downregulation promotes epithelial-to-mesenchymal transition.

ABSTRACT:

Background

Metastatic tumour cells are characterised by acquisition of migratory and invasive properties; properties shared by cells, which have undergone epithelial-to-mesenchymal transition (EMT). Disabled-2 (Dab2) is a putative tumour suppressor whose expression has been shown to be downregulated in various cancer types including breast cancer; however, its exact function in suppressing tumour initiation or progression is unclear.

Methods

Disabled-2 isoform expression was determined by RT-PCR analysis in human normal and breast tumour samples. Using shRNA-mediated technology, Dab2 was stably downregulated in two cell model systems representing nontumourigenic human mammary epithelial cells. These cells were characterised for expression of EMT markers by RT-PCR and western

SUBMITTER: Martin JC 

PROVIDER: S-EPMC2994233 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature