The role of macrophage migration inhibitory factor in ocular surface disease pathogenesis after chemical burn in the murine eye.

ABSTRACT:

Purpose

To evaluate the role of macrophage migration inhibitory factor (MIF) in the wound healing process following severe chemical burns to the ocular surface.

Methods

Chemical burning of the ocular surface was induced in mice (C57BL/6) via the application of 0.1 M NaOH. Macrophage migration inhibitory factor (MIF), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) mRNA expression in the ocular surface and lacrimal gland was evaluated via real-time reverse transcription PCR on days 2, 7, and 30 after induction of the chemical burn. The expression of MIF protein in the ocular surface and lacrimal gland was evaluated via western blot analysis. Immunohistochemical staining was conducted to detect MIF and vasculoendothelial growth factor in the cornea during the wound

SUBMITTER: Oh SY 

PROVIDER: S-EPMC2994759 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature