The mitotic arrest deficient protein MAD2B interacts with the clathrin light chain A during mitosis.

ABSTRACT:

Background

Although the mitotic arrest deficient protein MAD2B (MAD2L2) is thought to inhibit the anaphase promoting complex (APC) by binding to CDC20 and/or CDH1 (FZR1), its exact role in cell cycle control still remains to be established.

Methodology/principal findings

Using a yeast two-hybrid interaction trap we identified the human clathrin light chain A (CLTA) as a novel MAD2B binding protein. A direct interaction was established in mammalian cells via GST pull-down and endogenous co-immunoprecipitation during the G2/M phase of the cell cycle. Through subsequent confocal laser scanning microscopy we found that MAD2B and CLTA co-localize at the mitotic spindle. Clathrin forms a trimeric structure, i.e., the clathrin triskelion, consisting of three heavy chains (CLTC), ea

SUBMITTER: Medendorp K 

PROVIDER: S-EPMC2994903 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature