Maximization of loading and stability of ssDNA:iron oxide nanoparticle complexes formed through electrostatic interaction.

ABSTRACT: The use of inorganic nanoparticles (NPs) as vectors for the delivery of oligonucleotides for in vitro and in vivo applications is rapidly gaining momentum. Some of the reasons making them especially good candidates for this purpose are their ease of synthesis in a range of sizes and surface coatings, their propensity to penetrate cell membranes, their stability and biocompatibility, and their unique size-dependent physical properties that impart additional diagnostic and therapeutic tools. Notwithstanding these notable attributes, a major obstacle to their practical use is given by the typically low oligonucleotide loading levels attainable through conventional bioconjugation procedures. This shortcoming is especially worrisome as toxicity concerns have been associated with codelivery of N

SUBMITTER: Berti L 

PROVIDER: S-EPMC2994962 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature