Oriented insertion of phi29 N-hexahistidine-tagged gp10 connector protein assemblies into C20BAS bolalipid membrane vesicles.

ABSTRACT: Ni(2+):NTA-PEG600-grafted glass surfaces are capable of immobilizing N-his6 gp10 connector protein assemblies from the phi29 DNA packaging motor and mediating their transplantation into bolalipid vesicles whose membrane thickness is compatible with the hydrophobic domain of the gp10 assemblies.

SUBMITTER: Hyun SH 

PROVIDER: S-EPMC2995011 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature