The Trypanosoma brucei MitoCarta and its regulation and splicing pattern during development.

ABSTRACT: It has long been known that trypanosomes regulate mitochondrial biogenesis during the life cycle of the parasite; however, the mitochondrial protein inventory (MitoCarta) and its regulation remain unknown. We present a novel computational method for genome-wide prediction of mitochondrial proteins using a support vector machine-based classifier with ∼90% prediction accuracy. Using this method, we predicted the mitochondrial localization of 468 proteins with high confidence and have experimentally verified the localization of a subset of these proteins. We then applied a recently developed parallel sequencing technology to determine the expression profiles and the splicing patterns of a total of 1065 predicted MitoCarta transcripts during the development of the parasite, and showed that 435

SUBMITTER: Zhang X 

PROVIDER: S-EPMC2995047 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature