Rho Kinase Regulation of Fibroblast Migratory Mechanics in Fibrillar Collagen Matrices.

ABSTRACT: Migration of activated corneal fibroblasts plays an important role in matrix patterning during embryonic development and wound repopulation following injury or refractive surgery. In this study, we investigate the role of Rho kinase in regulating fibroblast migration mechanics, by modifying a previously described nested collagen matrix model to facilitate dynamic imaging of cell-matrix interactions.Human corneal fibroblasts were cultured in nested matrices with media containing either 1% fetal bovine serum (FBS), or 1% FBS plus the Rho kinase inhibitor Y-27632. Time-lapse DIC imaging of cell and extracellular matrix (ECM) movements was performed for up to 72 hours. In addition, static confocal imaging was used to assess 3-D cell morphology and local matrix reorganization.In 1% FBS, signifi

SUBMITTER: Zhou C 

PROVIDER: S-EPMC2995278 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature