Tumour auto-antibody screening: performance of protein microarrays using SEREX derived antigens.

ABSTRACT:

Background

The simplicity and potential of minimal invasive testing using serum from patients make auto-antibody based biomarkers a very promising tool for use in diagnostics of cancer and auto-immune disease. Although several methods exist for elucidating candidate-protein markers, immobilizing these onto membranes and generating so called macroarrays is of limited use for marker validation. Especially when several hundred samples have to be analysed, microarrays could serve as a good alternative since processing macro membranes is cumbersome and reproducibility of results is moderate.

Methods

Candidate markers identified by SEREX (serological identification of antigens by recombinant expression cloning) screenings of brain and lung tumour were used for macroarray and micro

SUBMITTER: Stempfer R 

PROVIDER: S-EPMC2995456 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature