Immunoassay for human serum hemojuvelin.

ABSTRACT:

Background

Hemojuvelin, a critical regulator of iron homeostasis, is involved in the regulation of hepcidin expression and iron homeostasis. It is expressed both as a membrane-bound form and as a soluble one. Serum hemojuvelin can be produced by secretion following furin cleavage or by proteolytic cleavage of the membrane-bound form by matriptase 2 (TMPRSS6). These forms contribute to down-regulation of hepcidin expression upon iron deficiency or hypoxia. This study describes the development and validation of the first enzyme-linked immunosorbent assay for hemojuvelin in human serum.

Design and methods

This assay is based on the use of a recombinant human repulsive guidance molecule-c peptide and a polyclonal antibody against hemojuvelin able to recognize the recombinant pep

SUBMITTER: Brasse-Lagnel C 

PROVIDER: S-EPMC2995560 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature