Adenovirus targeting to prostate-specific membrane antigen through virus-displayed, semirandom peptide library screening.

ABSTRACT: The convergence of phage-displayed peptide libraries and recombinant viral vectors launched a promising new direction in targeted viral gene therapeutics, but the translation of targeting peptides to functional cancer therapeutic agents has been challenging. Here, we report progress in developing a successful strategy to optimize targeted viral infection through adenovirus-displayed, semirandom peptide libraries. A phage-derived peptide targeting the prostate-specific membrane antigen (PSMA) was genetically incorporated into the adenoviral capsid Fiber protein and flanked by random peptide cassettes. The resulting adenovirus library was biopanned against PSMA-expressing cells and tumors to identify a PSMA-retargeted adenovirus. While the initial peptide alone could not target viral infecti

SUBMITTER: Wu P 

PROVIDER: S-EPMC2995819 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature