Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions.

ABSTRACT: Human induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells represent a promising unlimited cell source for generating patient-specific cells for biomedical research and personalized medicine. As a first step, critical to clinical applications, we attempted to develop defined culture conditions to expand and differentiate human iPSCs into functional progeny such as dopaminergic neurons for treating or modeling Parkinson's disease (PD). We used a completely defined (xeno-free) system that we previously developed for efficient generation of authentic dopaminergic neurons from human embryonic stem cells (hESCs), and applied it to iPSCs. First, we adapted two human iPSC lines derived from different somatic cell types for the defined expansion medium and showed that the iPSCs g

SUBMITTER: Swistowski A 

PROVIDER: S-EPMC2996088 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature