De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology.

ABSTRACT:

Background

Anopheles funestus is one of the primary vectors of human malaria, which causes a million deaths each year in sub-Saharan Africa. Few scientific resources are available to facilitate studies of this mosquito species and relatively little is known about its basic biology and evolution, making development and implementation of novel disease control efforts more difficult. The An. funestus genome has not been sequenced, so in order to facilitate genome-scale experimental biology, we have sequenced the adult female transcriptome of An. funestus from a newly founded colony in Burkina Faso, West Africa, using the Illumina GAIIx next generation sequencing platform.

Methodology/principal findings

We assembled short Illumina reads de novo using a novel approach involving i

SUBMITTER: Crawford JE 

PROVIDER: S-EPMC2996306 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature