Heart transcriptome of the bank vole (Myodes glareolus): towards understanding the evolutionary variation in metabolic rate.

ABSTRACT:

Background

Understanding the genetic basis of adaptive changes has been a major goal of evolutionary biology. In complex organisms without sequenced genomes, de novo transcriptome assembly using a longer read sequencing technology followed by expression profiling using short reads is likely to provide comprehensive identification of adaptive variation at the expression level and sequence polymorphisms in coding regions. We performed sequencing and de novo assembly of the bank vole heart transcriptome in lines selected for high metabolism and unselected controls.

Results

A single 454 Titanium run produced over million reads, which were assembled into 63,581 contigs. Searches against the SwissProt protein database and the ENSEMBL collection of mouse transcripts detected simila

SUBMITTER: Babik W 

PROVIDER: S-EPMC2996923 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature