Mobile element scanning (ME-Scan) by targeted high-throughput sequencing.

ABSTRACT:

Background

Mobile elements (MEs) are diverse, common and dynamic inhabitants of nearly all genomes. ME transposition generates a steady stream of polymorphic genetic markers, deleterious and adaptive mutations, and substrates for further genomic rearrangements. Research on the impacts, population dynamics, and evolution of MEs is constrained by the difficulty of ascertaining rare polymorphic ME insertions that occur against a large background of pre-existing fixed elements and then genotyping them in many individuals.

Results

Here we present a novel method for identifying nearly all insertions of a ME subfamily in the whole genomes of multiple individuals and simultaneously genotyping (for presence or absence) those insertions that are variable in the population. We use ME-s

SUBMITTER: Witherspoon DJ 

PROVIDER: S-EPMC2996938 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature