Recovery of the mitochondrial COI barcode region in diverse Hexapoda through tRNA-based primers.

ABSTRACT:

Background

DNA barcoding uses a 650 bp segment of the mitochondrial cytochrome c oxidase I (COI) gene as the basis for an identification system for members of the animal kingdom and some other groups of eukaryotes. PCR amplification of the barcode region is a key step in the analytical chain, but it sometimes fails because of a lack of homology between the standard primer sets and target DNA.

Results

Two forward PCR primers were developed following analysis of all known arthropod mitochondrial genome arrangements and sequence alignment of the tRNA-W gene which was usually located within 200 bp upstream of the COI gene. These two primers were combined with a standard reverse primer (LepR1) to produce a cocktail which generated a barcode amplicon from 125 of 141 species that i

SUBMITTER: Park DS 

PROVIDER: S-EPMC2996951 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature