Deep sequencing discovery of novel and conserved microRNAs in trifoliate orange (Citrus trifoliata).

ABSTRACT:

Background

MicroRNAs (miRNAs) play a critical role in post-transcriptional gene regulation and have been shown to control many genes involved in various biological and metabolic processes. There have been extensive studies to discover miRNAs and analyze their functions in model plant species, such as Arabidopsis and rice. Deep sequencing technologies have facilitated identification of species-specific or lowly expressed as well as conserved or highly expressed miRNAs in plants.

Results

In this research, we used Solexa sequencing to discover new microRNAs in trifoliate orange (Citrus trifoliata) which is an important rootstock of citrus. A total of 13,106,753 reads representing 4,876,395 distinct sequences were obtained from a short RNA library generated from small RNA extrac

SUBMITTER: Song C 

PROVIDER: S-EPMC2996959 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature