Microarray analysis of Shigella flexneri-infected epithelial cells identifies host factors important for apoptosis inhibition.

ABSTRACT:

Background

Shigella flexneri inhibits apoptosis in infected epithelial cells. In order to understand the pro-survival effects induced by the bacteria, we utilized apoptosis-specific microarrays to analyze the changes in eukaryotic gene expression in both infected and uninfected cells in the presence and absence of staurosporine, a chemical inducer of the intrinsic pathway of apoptosis. The goal of this research was to identify host factors that contribute to apoptosis inhibition in infected cells.

Results

The microarray analysis revealed distinct expression profiles in uninfected and infected cells, and these changes were altered in the presence of staurosporine. These profiles allowed us to make comparisons between the treatment groups. Compared to uninfected cells, Shigell

SUBMITTER: Faherty CS 

PROVIDER: S-EPMC2996966 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature