Genomic encyclopedia of sugar utilization pathways in the Shewanella genus.

ABSTRACT:

Background

Carbohydrates are a primary source of carbon and energy for many bacteria. Accurate projection of known carbohydrate catabolic pathways across diverse bacteria with complete genomes constitutes a substantial challenge due to frequent variations in components of these pathways. To address a practically and fundamentally important challenge of reconstruction of carbohydrate utilization machinery in any microorganism directly from its genomic sequence, we combined a subsystems-based comparative genomic approach with experimental validation of selected bioinformatic predictions by a combination of biochemical, genetic and physiological experiments.

Results

We applied this integrated approach to systematically map carbohydrate utilization pathways in 19 genomes from th

SUBMITTER: Rodionov DA 

PROVIDER: S-EPMC2996990 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature