Study of inter- and intra-individual variations in the salivary microbiota.

ABSTRACT:

Background

Oral bacterial communities contain species that promote health and others that have been implicated in oral and/or systemic diseases. Culture-independent approaches provide the best means to assess the diversity of oral bacteria because most of them remain uncultivable.

Results

The salivary microbiota from five adults was analyzed at three time-points by means of the 454 pyrosequencing technology. The V1-V3 region of the bacterial 16S rRNA genes was amplified by PCR using saliva lysates and broad-range primers. The bar-coded PCR products were pooled and sequenced unidirectionally to cover the V3 hypervariable region. Of 50,708 obtained sequences, 31,860 passed the quality control. Non-bacterial sequences (2.2%) were removed leaving 31,170 reads. Samples were domin

SUBMITTER: Lazarevic V 

PROVIDER: S-EPMC2997015 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature