Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.

ABSTRACT:

Background

Nesfatin-1 is a recently discovered anorexigen encoded in the precursor peptide, nucleobindin-2 (NUCB2) in mammals. To date, nesfatin-1 has not been described in any non-mammalian species, although some information is available in the sequenced genomes of several species. Our objective was to characterize nesfatin-1 in fish.

Methodology/principal findings

In the present study, we employed molecular, immunohistochemical, and physiological studies to characterize the structure, distribution, and appetite regulatory effects of nesfatin-1 in a non-mammalian vertebrate. A very high conservation in NUCB2 sequences, especially in the nesfatin-1 region was found in lower vertebrates. Abundant expression of NUCB2 mRNA was detected in several tissues including the brain and

SUBMITTER: Gonzalez R 

PROVIDER: S-EPMC2997068 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature