Molecular epidemiology of Plasmodium species prevalent in Yemen based on 18 s rRNA.

ABSTRACT:

Background

Malaria is an endemic disease in Yemen and is responsible for 4.9 deaths per 100,000 population per year and 43,000 disability adjusted life years lost. Although malaria in Yemen is caused mainly by Plasmodium falciparum and Plasmodium vivax, there are no sequence data available on the two species. This study was conducted to investigate the distribution of the Plasmodium species based on the molecular detection and to study the molecular phylogeny of these parasites.

Methods

Blood samples from 511 febrile patients were collected and a partial region of the 18 s ribosomal RNA (18 s rRNA) gene was amplified using nested PCR. From the 86 positive blood samples, 13 Plasmodium falciparum and 4 Plasmodium vivax were selected and underwent cloning and, subsequently, seq

SUBMITTER: Al-Mekhlafi AM 

PROVIDER: S-EPMC2997089 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature