Dried blood spot real-time polymerase chain reaction assays to screen newborns for congenital cytomegalovirus infection.

ABSTRACT:

Context

Reliable methods to screen newborns for congenital cytomegalovirus (CMV) infection are needed for identification of infants at increased risk of hearing loss. Since dried blood spots (DBS) are routinely collected for metabolic screening from all newborns in the United States, there has been interest in using DBS polymerase chain reaction (PCR)-based methods for newborn CMV screening.

Objective

To determine the diagnostic accuracy of DBS real-time PCR assays for newborn CMV screening.

Design, setting, and participants

Between March 2007 and May 2008, infants born at 7 US medical centers had saliva specimens tested by rapid culture for early antigen fluorescent foci. Results of saliva rapid culture were compared with a single-primer (March 2007-December 2007) an

SUBMITTER: Boppana SB 

PROVIDER: S-EPMC2997517 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature