Genetic engineering of the glyoxalase pathway in tobacco leads to enhanced salinity tolerance.

ABSTRACT: The glyoxalase pathway involving glyoxalase I (gly I) and glyoxalase II (gly II) enzymes is required for glutathione-based detoxification of methylglyoxal. We had earlier indicated the potential of gly I as a probable candidate gene in conferring salinity tolerance. We report here that overexpression of gly I+II together confers improved salinity tolerance, thus offering another effective strategy for manipulating stress tolerance in crop plants. We have overexpressed the gly II gene either alone in untransformed plants or with gly I transgenic background. Both types of these transgenic plants stably expressed the foreign protein, and the enzyme activity was also higher. Compared with nontransformants, several independent gly II transgenic lines showed improved capability for tolerating ex

SUBMITTER: Singla-Pareek SL 

PROVIDER: S-EPMC299757 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature