A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses.

ABSTRACT: Acidobacteria are ubiquitous and abundant members of soil bacterial communities. However, an ecological understanding of this important phylum has remained elusive because its members have been difficult to culture and few molecular investigations have focused exclusively on this group. We generated an unprecedented number of acidobacterial DNA sequence data using pyrosequencing and clone libraries (39,707 and 1787 sequences, respectively) to characterize the relative abundance, diversity and composition of acidobacterial communities across a range of soil types. To gain insight into the ecological characteristics of acidobacterial taxa, we investigated the large-scale biogeographic patterns exhibited by acidobacterial communities, and related soil and site characteristics to acidobacteria

SUBMITTER: Jones RT 

PROVIDER: S-EPMC2997719 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature