Discovery of mutations in Saccharomyces cerevisiae by pooled linkage analysis and whole-genome sequencing.

ABSTRACT: Many novel and important mutations arise in model organisms and human patients that can be difficult or impossible to identify using standard genetic approaches, especially for complex traits. Working with a previously uncharacterized dominant Saccharomyces cerevisiae mutant with impaired vacuole inheritance, we developed a pooled linkage strategy based on next-generation DNA sequencing to specifically identify functional mutations from among a large excess of polymorphisms, incidental mutations, and sequencing errors. The VAC6-1 mutation was verified to correspond to PHO81-R701S, the highest priority candidate reported by VAMP, the new software platform developed for these studies. Sequence data further revealed the large extent of strain background polymorphisms and structural alteration

SUBMITTER: Birkeland SR 

PROVIDER: S-EPMC2998298 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature