Exploiting natural variation in Saccharomyces cerevisiae to identify genes for increased ethanol resistance.

ABSTRACT: Ethanol production from lignocellulosic biomass holds promise as an alternative fuel. However, industrial stresses, including ethanol stress, limit microbial fermentation and thus prevent cost competitiveness with fossil fuels. To identify novel engineering targets for increased ethanol tolerance, we took advantage of natural diversity in wild Saccharomyces cerevisiae strains. We previously showed that an S288c-derived lab strain cannot acquire higher ethanol tolerance after a mild ethanol pretreatment, which is distinct from other stresses. Here, we measured acquired ethanol tolerance in a large panel of wild strains and show that most strains can acquire higher tolerance after pretreatment. We exploited this major phenotypic difference to address the mechanism of acquired ethanol toleran

SUBMITTER: Lewis JA 

PROVIDER: S-EPMC2998304 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature