Liquid-liquid phase separation of a monoclonal antibody and nonmonotonic influence of Hofmeister anions.

ABSTRACT: Liquid-liquid phase separation was studied for a monoclonal antibody in the monovalent salt solutions of KF, KCl, and KSCN under different pH conditions. A modified Carnahan-Starling hard-sphere model was utilized to fit the experimental data, establish the liquid-liquid coexistence curve, and determine antibody-antibody interactions in the form of T(c) (critical temperature) under the different solution conditions. The liquid-liquid phase separation revealed the complex relationships between antibody-antibody interactions and different solution conditions, such as pH, ionic strength, and the type of anion. At pH 7.1, close to the pI of the antibody, a decrease of T(c) versus ionic strength was observed at low salt conditions, suggesting that the protein-protein interactions became less at

SUBMITTER: Mason BD 

PROVIDER: S-EPMC2998619 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature