Distinguishable populations report on the interactions of single DNA molecules with solid-state nanopores.

ABSTRACT: Solid-state nanopores have received increasing interest over recent years because of their potential for genomic screening and sequencing. In particular, small nanopores (2-5 nm in diameter) allow the detection of local structure along biological molecules, such as proteins bound to DNA or possibly the secondary structure of RNA molecules. In a typical experiment, individual molecules are translocated through a single nanopore, thereby causing a small deviation in the ionic conductance. A correct interpretation of these conductance changes is essential for our understanding of the process of translocation, and for further sophistication of this technique. Here, we present translocation measurements of double-stranded DNA through nanopores down to the diameter of the DNA itself (1.8-7 nm at

SUBMITTER: van den Hout M 

PROVIDER: S-EPMC2998632 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature