Relationships linking amplification level to gene over-expression in gliomas.

ABSTRACT:

Background

Gene amplification is thought to promote over-expression of genes favouring tumour development. Because amplified regions are usually megabase-long, amplification often concerns numerous syntenic or non-syntenic genes, among which only a subset is over-expressed. The rationale for these differences remains poorly understood.

Methodology/principal finding

To address this question, we used quantitative RT-PCR to determine the expression level of a series of co-amplified genes in five xenografted and one fresh human gliomas. These gliomas were chosen because we have previously characterised in detail the genetic content of their amplicons. In all the cases, the amplified sequences lie on extra-chromosomal DNA molecules, as commonly observed in gliomas. We show here t

SUBMITTER: Vogt N 

PROVIDER: S-EPMC2999539 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature