High-temperature gas chromatography-mass spectrometry for skin surface lipids profiling.

ABSTRACT: Skin surface lipids (SSLs) arising from both sebaceous glands and skin removal form a complex lipid mixture composed of free fatty acids and neutral lipids. High-temperature gas chromatography coupled with electron impact or chemical ionization mass spectrometry was used to achieve a simple analytical protocol, without prior separation in classes and without prior cleavage of lipid molecules, in order to obtain simultaneously i) a qualitative characterization of the individual SSLs and ii) a quantitative evaluation of lipid classes. The method was first optimized with SSLs collected from the forehead of a volunteer. More than 200 compounds were identified in the same run. These compounds have been classified in five lipid classes: free fatty acids, hydrocarbons, waxes, sterols, and glyceri

SUBMITTER: Michael-Jubeli R 

PROVIDER: S-EPMC2999924 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature