Total testosterone assays in women with polycystic ovary syndrome: precision and correlation with hirsutism.

ABSTRACT:

Context

There is no standardized assay of testosterone in women. Liquid chromatography mass spectrometry (LC/MS) has been proposed as the preferable assay by an Endocrine Society Position Statement.

Objective

The aim was to compare assay results from a direct RIA with two LC/MS.

Design and setting

We conducted a blinded laboratory study including masked duplicate samples at three laboratories--two academic (University of Virginia, RIA; and Mayo Clinic, LC/MS) and one commercial (Quest, LC/MS).

Participants and interventions

Baseline testosterone levels from 596 women with PCOS who participated in a large, multicenter, randomized controlled infertility trial performed at academic health centers in the United States were run by varying assays, and results were co

SUBMITTER: Legro RS 

PROVIDER: S-EPMC2999971 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature