Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina.

ABSTRACT:

Purpose

The CERKL gene encodes for ceramide kinase-like, a novel protein of unknown function. CERKL mutations are associated with a severe retinal phenotype. The purpose of this work was to investigate alternative splicing, and the temporal and spatial expression pattern of CERKL in the mouse retina.

Methods

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) analysis of mouse retina RNA was used to study the expression of Cerkl at various developmental time points, and to identify its various splice-isoforms. A specific anti-CERKL antibody was developed and used for immunostaining to study the localization of the endogenous CERKL protein in retina-derived cell lines and in the mouse retina.

Results

Cerkl is expressed in the mouse eye as early as embryonic day 14

SUBMITTER: Vekslin S 

PROVIDER: S-EPMC3000240 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature