Differential correction of lagging-strand replication errors made by DNA polymerases {alpha} and {delta}.

ABSTRACT: Mismatch repair (MMR) of replication errors requires DNA ends that can direct repair to the newly synthesized strand containing the error. For all but those organisms that use adenine methylation to generate nicks, the source of these ends in vivo is unknown. One possibility is that MMR may have a "special relation to the replication complex" [Wagner R, Jr., Meselson M (1976) Proc Natl Acad Sci USA 73:4135-4139], perhaps one that allows 5' or 3' DNA ends associated with replication to act as strand discrimination signals. Here we examine this hypothesis, based on the logic that errors made by yeast DNA polymerase α (Pol α), which initiates Okazaki fragments during lagging-strand replication, will always be closer to a 5' end than will be more internal errors generated by DNA polymerase δ (

SUBMITTER: Nick McElhinny SA 

PROVIDER: S-EPMC3000245 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature