Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.

ABSTRACT:

Background

A variant of the CDKAL1 gene was reported to be associated with type 2 diabetes and reduced insulin release in humans; however, the role of CDKAL1 in β cells is largely unknown. Therefore, to determine the role of CDKAL1 in insulin release from β cells, we studied insulin release profiles in CDKAL1 gene knockout (CDKAL1 KO) mice.

Principal findings

Total internal reflection fluorescence imaging of CDKAL1 KO β cells showed that the number of fusion events during first-phase insulin release was reduced. However, there was no significant difference in the number of fusion events during second-phase release or high K(+)-induced release between WT and KO cells. CDKAL1 deletion resulted in a delayed and slow increase in cytosolic free Ca(2+) concentration during high gl

SUBMITTER: Ohara-Imaizumi M 

PROVIDER: S-EPMC3000340 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature