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Counting single molecules in sub-nanolitre droplets.

ABSTRACT: We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

SUBMITTER: Rane TD 

PROVIDER: S-EPMC3000353 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Counting single molecules in sub-nanolitre droplets.

Rane T D TD   Puleo C M CM   Liu K J KJ   Zhang Y Y   Lee A P AP   Wang T H TH  

Lab on a chip 20091009 2

We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction. ...[more]

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