Single-molecule adhesion forces and attachment lifetimes of myosin-I phosphoinositide interactions.

ABSTRACT: Phosphoinositides regulate the activities and localization of many cytoskeletal proteins involved in crucial biological processes, including membrane-cytoskeleton adhesion. Yet little is known about the mechanics of protein-phosphoinositide interactions, or about the membrane-attachment mechanics of any peripheral membrane proteins. Myosin-Ic (myo1c) is a molecular motor that links membranes to the cytoskeleton via phosphoinositide binding, so it is particularly important to understand the mechanics of its membrane attachment. We used optical tweezers to measure the strength and attachment lifetime of single myo1c molecules as they bind beads coated with a bilayer of 2% phosphatidylinositol 4,5-bisphosphate and 98% phosphatidylcholine. Adhesion forces measured under ramp-load ranged betwee

SUBMITTER: Pyrpassopoulos S 

PROVIDER: S-EPMC3000492 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature