Kita driven expression of oncogenic HRAS leads to early onset and highly penetrant melanoma in zebrafish.

ABSTRACT:

Background

Melanoma is the most aggressive and lethal form of skin cancer. Because of the increasing incidence and high lethality of melanoma, animal models for continuously observing melanoma formation and progression as well as for testing pharmacological agents are needed.

Methodology and principal findings

Using the combinatorial Gal4-UAS system, we have developed a zebrafish transgenic line that expresses oncogenic HRAS under the kita promoter. Already at 3 days transgenic kita-GFP-RAS larvae show a hyper-pigmentation phenotype as earliest evidence of abnormal melanocyte growth. By 2-4 weeks, masses of transformed melanocytes form in the tail stalk of the majority of kita-GFP-RAS transgenic fish. The adult tumors evident between 1-3 months of age faithfully reproduce th

SUBMITTER: Santoriello C 

PROVIDER: S-EPMC3000817 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature