Quantitating antibody uptake in vivo: conditional dependence on antigen expression levels.

ABSTRACT:

Purpose

Antibodies form an important class of cancer therapeutics, and there is intense interest in using them for imaging applications in diagnosis and monitoring of cancer treatment. Despite the expanding body of knowledge describing pharmacokinetic and pharmacodynamic interactions of antibodies in vivo, discrepancies remain over the effect of antigen expression level on tumoral uptake with some reports indicating a relationship between uptake and expression and others showing no correlation.

Procedures

Using a cell line with high epithelial cell adhesion molecule expression and moderate epidermal growth factor receptor expression, fluorescent antibodies with similar plasma clearance were imaged in vivo. A mathematical model and mouse xenograft experiments were used to des

SUBMITTER: Thurber GM 

PROVIDER: S-EPMC3000888 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature