Mapping of long-range associations throughout the fission yeast genome reveals global genome organization linked to transcriptional regulation.

ABSTRACT: We have comprehensively mapped long-range associations between chromosomal regions throughout the fission yeast genome using the latest genomics approach that combines next generation sequencing and chromosome conformation capture (3C). Our relatively simple approach, referred to as enrichment of ligation products (ELP), involves digestion of the 3C sample with a 4 bp cutter and self-ligation, achieving a resolution of 20 kb. It recaptures previously characterized genome organizations and also identifies new and important interactions. We have modeled the 3D structure of the entire fission yeast genome and have explored the functional relationships between the global genome organization and transcriptional regulation. We find significant associations among highly transcribed genes. Moreove

SUBMITTER: Tanizawa H 

PROVIDER: S-EPMC3001101 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature