Host-derived interleukin-5 promotes adenocarcinoma-induced malignant pleural effusion.

ABSTRACT:

Rationale

IL-5 is a T helper 2 cytokine important in the trafficking and survival of eosinophils. Because eosinophils can be found in malignant pleural effusions (MPE) from mice and humans, we asked whether IL-5 is involved in the pathogenesis of MPE.

Objectives

To determine the role of IL-5 in MPE formation.

Methods

The effects of IL-5 on experimental MPE induced in C57BL/6 mice by intrapleural injection of syngeneic lung (Lewis lung cancer [LLC]) or colon (MC38) adenocarcinoma cells were determined using wild-type (il5(+/+)) and IL-5-deficient (il5⁻(/)⁻) mice, exogenous administration of recombinant mouse (rm) IL-5, and in vivo antibody-mediated neutralization of endogenous IL-5. The direct effects of rmIL-5 on LLC cell proliferation and gene expression in vitro wer

SUBMITTER: Stathopoulos GT 

PROVIDER: S-EPMC3001265 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature